1347 Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of skin biology, but its utility is restricted by the requirement fresh samples, inadequate dissociation-induced cell loss or death, and activation during tissue digestion. Here, we profiled cells using single-nucleus (snRNA-seq) in parallel with scRNA-seq. We found that snRNA-seq identified more spatially functionally relevant keratinocyte clusters constitute trajectories expected differentiation dynamics. Novel markers, e.g., LYPD3, EMP2, CSTB, were revealed for different stage keratinocytes, NFIB GRHL1 as transcription factors involving proliferation functional keratinocytes. Fibroblasts a state scRNA-seq, demonstrating distinct transcriptomic features contrast to those snRNA-seq. scRNA-seq detected greater number immune cells, especially many activated types. Moreover, analysis on strength inferred interactions among fibroblasts core cell-to-cell interactions. Ligands COLLAGEN, LAMININ, SEMA3 signaling pathways enriched snRNA-seq, indicating an important role maintenance homeostasis. Overall, generated updated atlas transcriptome based reciprocal contribution